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human fetal lung fibroblast wi 38  (ATCC)


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    ATCC human fetal lung fibroblast wi 38
    Human Fetal Lung Fibroblast Wi 38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fetal lung fibroblast wi 38/product/ATCC
    Average 99 stars, based on 3638 article reviews
    human fetal lung fibroblast wi 38 - by Bioz Stars, 2026-03
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    human fetal lung fibroblast wi 38  (ATCC)
    99
    ATCC human fetal lung fibroblast wi 38
    Human Fetal Lung Fibroblast Wi 38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fetal lung fibroblast wi 38/product/ATCC
    Average 99 stars, based on 1 article reviews
    human fetal lung fibroblast wi 38 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    human fetal lung fibroblasts  (ATCC)
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    ATCC human fetal lung fibroblasts
    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung <t>fibroblasts</t> (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.
    Human Fetal Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fetal lung fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    human fetal lung fibroblasts - by Bioz Stars, 2026-03
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    culture system human fetal lung fibroblasts  (ATCC)
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    ATCC culture system human fetal lung fibroblasts
    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung <t>fibroblasts</t> (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.
    Culture System Human Fetal Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/culture system human fetal lung fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    culture system human fetal lung fibroblasts - by Bioz Stars, 2026-03
    99/100 stars
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    fetal human lung fibroblasts  (ATCC)
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    ATCC fetal human lung fibroblasts
    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung <t>fibroblasts</t> (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.
    Fetal Human Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal human lung fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    fetal human lung fibroblasts - by Bioz Stars, 2026-03
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    wi-38  (ATCC)
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    ATCC wi-38
    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung <t>fibroblasts</t> (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.
    Wi 38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wi-38/product/ATCC
    Average 99 stars, based on 1 article reviews
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    wi38 human fetal lung fibroblasts  (ATCC)
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    ATCC wi38 human fetal lung fibroblasts
    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung <t>fibroblasts</t> (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.
    Wi38 Human Fetal Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wi38 human fetal lung fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    wi38 human fetal lung fibroblasts - by Bioz Stars, 2026-03
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    AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung fibroblasts (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.

    Journal: Molecular Therapy Oncology

    Article Title: Tackling cancer heterogeneity with systemically delivered oncolytic adenoviruses transcriptionally targeted with hybrid promoters

    doi: 10.1016/j.omton.2025.201073

    Figure Lengend Snippet: AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung fibroblasts (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.

    Article Snippet: Human CRC cell lines (LoVo, T84, HCT116, and HT29), normal colon epithelial cells (CCD841), human embryonic kidney cells (HEK293), human fetal lung fibroblasts (WI-38), and human microendothelial cells (HMEC-1) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Activity Assay, In Vitro, Positive Control, Infection, MTS Assay, Control, Expressing, Western Blot